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anti cd31  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti cd31
    Anti Cd31, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31/pmc12992974-282-65-67
    Average 86 stars, based on 1 article reviews
    anti cd31 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: MEG3 in glioma stem cells promotes glioblastoma angiogenesis through FUBP3-mediated VGF expression.
    Article Snippet: Signal detection was performed using the ChemiDoc Imaging System (BIO-RAD). .. The primary antibodies used for immunoblotting included VGF (Abcam, ab313783, 1:1000 dilution), β-actin (Proteintech, 66009-1-Ig, 1:5000 dilution), FUBP3 (Proteintech, 10623-1-AP, 1:1000 dilution), OLIG2 (Abcam, ab109186, 1:1000 dilution), SOX2 (Cell Signaling Technology, 3579, 1:2000 dilution), GFAP (Biolegend, PRB-571C, 1:1000 dilution), and CD31 (CST, 77699, 1:1000 dilution). .. Total RNA was extracted from T4121 vector/MEG3OE, T4121 shNT/shMEG3#1/ shMEG3#2 cells using TRIzol Reagent (Invitrogen, 15596026) according to the manufacturer’s instructions.

    Staining:

    Article Title: PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth
    Article Snippet: .. Tumor sections were stained with antibodies against HBsAg (Creative Diagnostics, #DMABT–51328MH) and CD31 (Cell Signaling Technology, #77699). .. Images were captured using an Olympus BX53 microscope (Olympus).

    Article Title: PRMT5-mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth.
    Article Snippet: .. Tumor sections were stained with antibodies against HBsAg (Creative Diagnostics, #DMABT–51328MH) and CD31 (Cell Signaling Technology, #77699). .. Images were captured using an Olympus BX53 microscope (Olympus).

    Incubation:

    Article Title: MEG3 in glioma stem cells promotes glioblastoma angiogenesis through FUBP3-mediated VGF expression
    Article Snippet: .. The sections were incubated overnight at 4°C with primary antibodies targeting VGF (Abcam, ab313783, 1:250 dilution) and CD31 (Cell Signaling Technology, 3579, 1:200 dilution). .. After washing, the sections were treated with avidin-biotin-peroxidase (MXB Biotechnologies, KIT-5220) for 15 min at room temperature, and visualization was performed using diaminobenzidine (MXB Biotechnologies, KIT-5220) for 1–2 min.

    Article Title: Tailored Porous Bimetallic Nanozyme Platform for Full-Cycle Therapeutics of Intestinal Ischemia/Reperfusion.
    Article Snippet: .. Blocking treatments were performed, and the sections were incubated overnight at 4◦C with primary antibodies against CD86 (Proteintech, 13395-1-AP, 1:200, RRID:AB_2074882), CD206 (Cell Signaling Technology, 24595S, 1:100), α-SMA (Proteintech, 14395-1-AP, 1:200, RRID:AB_2223009), CD31 (Cell Signaling Technology, 15585T, 1:200), and Occludin (Abcam, ab216327, 1:200, RRID:AB_2737295). .. The next day, after washing with PBS, the sections were incubated with Alexa Fluor 594 or Alexa Fluor 488-labeled secondary antibodies at room temperature in the dark for 30 min. DAPI (Beyotime Biotechnology, C1099, China) counterstaining was used to visualize the nuclei.

    Blocking Assay:

    Article Title: Tailored Porous Bimetallic Nanozyme Platform for Full-Cycle Therapeutics of Intestinal Ischemia/Reperfusion.
    Article Snippet: .. Blocking treatments were performed, and the sections were incubated overnight at 4◦C with primary antibodies against CD86 (Proteintech, 13395-1-AP, 1:200, RRID:AB_2074882), CD206 (Cell Signaling Technology, 24595S, 1:100), α-SMA (Proteintech, 14395-1-AP, 1:200, RRID:AB_2223009), CD31 (Cell Signaling Technology, 15585T, 1:200), and Occludin (Abcam, ab216327, 1:200, RRID:AB_2737295). .. The next day, after washing with PBS, the sections were incubated with Alexa Fluor 594 or Alexa Fluor 488-labeled secondary antibodies at room temperature in the dark for 30 min. DAPI (Beyotime Biotechnology, C1099, China) counterstaining was used to visualize the nuclei.



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    Image Search Results


    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Journal: Bioactive Materials

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    doi: 10.1016/j.bioactmat.2026.04.004

    Figure Lengend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Article Snippet: After antigen retrieval and blocking, sections were incubated overnight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789 ).

    Techniques: Immunofluorescence, Expressing, Marker